Anti- HLTF Antibody

Product name : Anti- HLTF Antibody

Catalog number : GEN2524129

Supplier : MBS Polyclonals

Price : 265 EUR

Size : 0.06 ml

More about:

Concept : Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

Discovery year : 2011-07-28

Entrez Gene : helicase like transcription factor  (HLTF)

Gene : HLTF-AS1

Gene ontology - Biological process : regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter covalent chromatin modification protein ubiquitination protein polyubiquitination transcription from RNA polymerase II promoter postreplication repair

Gene ontology - Cellular component : nucleus cytoplasm nucleoplasm membrane nucleolus

Gene ontology - Molecular function : protein binding zinc ion binding ATP binding DNA binding RNA binding helicase activity ATPase activity RNA polymerase II core promoter proximal region sequence-specific DNA binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding ubiquitin protein ligase activity

Havana BLAST/BLAT : OTTHUMG00000159531

Identity : 40554

InterPro : P-loop containing nucleoside triphosphate hydrolase Zinc finger, RING/FYVE/PHD-type  (MTMR3) Zinc finger, RING-type  (RNF113A) Zinc finger, RING-type, conserved site Helicase, C-terminal  (BRIP1) Helicase superfamily 1/2, ATP-binding domain  (VPS9D1) SNF2-related, N-terminal domain  (SMARCA4) Helicase-like transcription factor  (HLTF) HIRAN domain

Locus : 3q24

Long gene name : HLTF antisense RNA 1

Name : Blotting, Western

PubMed : HLTF and SHPRH are functional homologues of yeast Rad5 that cooperatively mediate PCNA polyubiquitination and maintain genomic stability. We were able to provide evidence that methylation of HLTF and especially HPP1 detected in serum is strongly correlated with cell death in CRC using LDH as surrogate marker HLTF/SMARCA3, a member of SWI/SNF family, is a common target for methylation and epigenetic gene silencing in colon cancer and a candidate colon cancer suppressor gene. These results suggest that HLTF promoter hypermethylation is frequently demonstrated in human gastric cancer, and inactivation of HLTF or the chromatin-remodeling complex may play a crucial role in gastric carcinogenesis. HLTF can promote error-free replication of damaged DNA and support a role for HLTF in preventing mutagenesis and carcinogenesis, providing thereby for its potential tumor suppressor role. HLTF can displace a broad spectrum of proteins such as replication protein A (RPA), PCNA, and replication factor C (RFC), thereby providing the first example for a protein clearing activity at the stalled replication fork. Results demonstrate that loss of HLTF function promotes the malignant transformation of intestinal or colonic adenomas to carcinomas by inducing genomic instability. Findings indicate a mechanism of helicase-like transcription factor HLTF-mediated fork reversal and suggest the requirement for distinct fork remodeling activities in the cell. HLTF promotes the filling-in of gaps left opposite damaged DNA during replication, and this postreplication repair function depends on its HIRAN domain. Fasudil reduced LPS-mediated TF and PAI-1 expression and activity in PBMCs. These effects may partially be relevant to the clinical benefits of fasudil in the treatment of CAPD patients.

Qualifiers : classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics

Synonyms gene name : HLTF antisense RNA 1 (non-protein coding)

Tree numbers : E05.196.401.143 E05.301.300.096 E05.478.566.320.200 E05.601.262 E05.601.470.320.200

WikiPathWays : Retinoblastoma (RB) in Cancer