ARHGEF7 Antibody

Product name : ARHGEF7 Antibody

Catalog number : CSB-PA01445A0Rb_50ug

Supplier : Cusabio

Price : 212 EUR

Size : 50ug

More about:

Alias : Beta-Pix,COOL-1,PAK-interacting exchange factor beta,p85,ARHGEF7,COOL1, KIAA0142, P85SPR, PAK3BP, PIXB ORF Names: Nbla10314

Antibody category : Polyclonal Antibodies

Background : Acts as a RAC1 guanine nucleotide exchange factor (GEF) and can induce membrane ruffling. Functions in cell migration, attachment and cell spreading. Promotes targeting of RAC1 to focal adhesions By similarity. May function as a positive regulator of apoptosis. Downstream of NMDA receptors and CaMKK-CaMK1 signaling cascade, promotes the formation of spines and synapses in hippocampal neurons.

Buffer composition : Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Concept : Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.

Conjugation : Non-conjugated

Discovery year : 2011-05-05

Entrez Gene : Rho guanine nucleotide exchange factor 7  (ARHGEF7)

Gene : ARHGEF7-AS1

Gene ontology - Biological process : signal transduction positive regulation of growth hormone secretion positive regulation of GTPase activity nervous system development regulation of small GTPase mediated signal transduction positive regulation of substrate adhesion-dependent cell spreading regulation of Rho protein signal transduction focal adhesion assembly regulation of GTP binding positive regulation of lamellipodium morphogenesis

Gene ontology - Cellular component : cytosol plasma membrane focal adhesion protein complex neuron projection neuronal cell body cell cortex lamellipodium ruffle storage vacuole

Gene ontology - Molecular function : protein binding protein kinase binding guanyl-nucleotide exchange factor activity Rho guanyl-nucleotide exchange factor activity

Havana BLAST/BLAT : OTTHUMG00000017355

Identity : 39816

Immunogen type : Recombinant Human Rho guanine nucleotide exchange factor 7 protein (1-250AA)

InterPro : Pleckstrin homology domain  (PLEKHA7) PH domain-like  (AGAP4) SH3 domain  (SBK1) Calponin homology domain  (LRCH1) Variant SH3 domain  (ITSN1) Dbl homology (DH) domain Guanine-nucleotide dissociation stimulator, CDC24, conserved site Rho guanine nucleotide exchange factor, coiled-coil domain

Kegg : Regulation of actin cytoskeleton - Homo sapiens (human)

Locus : 13q34

Long gene name : ARHGEF7 antisense RNA 1

Long name : Rabbit Antibody against Human ARHGEF7, Non-conjugated

Name : Blotting, Western

PubMed : Data show that Rac1 plays a significant role in the pathological adhesion of Schwannoma cells. Rac1 regulates nitric oxide synthase type 2 (NOD2) trafficking and NOD2-dependent signal transduction in primary human monocytes and cell line THP-1. the interaction of the betaPIX.WASP.SPIN90 complex with Nck is crucial for stable cell adhesion and can be dynamically modulated by SPIN90 phosphorylation that is dependent on cell adhesion and ERK activation These data strongly suggest that, in addition to the known SAP-interacting kinase Fyn, PIX may be another key player in SAP-mediated T cell activation. These results identify p66Shc and FOXO3a as novel partners of beta(1)Pix and represent the first direct evidence of beta(1)Pix in cell proliferation via Erk/p66Shc-dependent and Akt-independent mechanisms. betaPix up-regulates NHE3 membrane expression and activity by Shank2-mediated protein-protein interaction and by activating Rho GTPases in the apical regions of epithelial cells Downstream effects of phosphorylation of ARHGEF7 through LRRK2 could be (i) a feedback control mechanism for LRRK2 activity as well as (ii) an impact of LRRK2 on actin cytoskeleton regulation a model by which SNX27 regulates trafficking of beta-Pix to focal adhesions and thereby influences cell motility. beta1Pix functions as a transcriptional regulator of beta-catenin signaling through direct interaction with beta-catenin, an action that may be functionally relevant to colon cancer biology. Data indicate that suppression of c-Cbl protein by rho guanine nucleotide exchange factor 7 (Cool-1) may be critical for generation of at least a subset of glioblastoma (GBM).

Purification method : Protein G purified

Qualifiers : classification, economics, history, instrumentation, methods, standards, trends, utilization, veterinary, statistics & numerical data, ethics

Species reactivity : Human

Synonym name : ARHGEF7 Human Antibody

Synonyms gene name : ARHGEF7 antisense RNA 1 (non-protein coding)

Tested applications & recommended dilutions : ELISA,WB;Recommended dilution:WB:1:1000-1:5000

Tree numbers : E05.196.401.143 E05.301.300.096 E05.478.566.320.200 E05.601.262 E05.601.470.320.200

WikiPathWays : Integrin-mediated Cell Adhesion Regulation of Actin Cytoskeleton